rabbit anti-col1 ab  (ABclonal Biotechnology)

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet: Rv1509 suppresses osteoblast differentiation and mineralization (A) ALP staining and subsequent quantification were conducted on days 7 and 14 while utilizing osteogenic differentiation medium. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗, p < 0.05; ∗∗, p < 0.01; [Student’s t test]). (B) Alizarin red staining and quantification were executed on days 14 and 21 in the context of osteogenic differentiation medium. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (C) RT-qPCR analysis was carried out to evaluate the expression levels of osteogenic genes in neonatal mouse calvarial cells that were treated with Rv1509 on days 1, 3, 7, and 14. The data are shown as mean ± SEM. ( n = 3; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (D) Representative western blots of Runx2, Osterix, and Col1 proteins in osteoblasts after Rv1509 treatment.

Article Snippet: Rabbit anti-Col1 Ab , ABclonal , Cat# A22089;RRID: AB_3665644.

Techniques: Staining, Quantitative RT-PCR, Expressing, Western Blot

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet: Rv1509 indirectly suppressed osteoblast differentiation by enhancing the release of inflammatory factors from osteoclasts (A) Alizarin red staining and quantitative assessment of osteoblasts cultured for a duration of 21 days with supernatants derived from both control and Rv1509-treated osteoclasts. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗, p < 0.05 [Student’s t test]). (B) Osteoblasts were subjected to treatment for 14 days with culture supernatants from control and Rv1509-treated osteoclasts, followed by ALP staining and subsequent quantification. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (C) The expression of osteoblast-specific mRNA was analyzed using RT-qPCR after a 7-day culture period with supernatants from control and Rv1509-treated osteoclasts. The data are shown as mean ± SEM. ( n = 3; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (D) ELISA was conducted to quantify the expression levels of inflammatory factors in osteoclasts from both the control and Rv1509-treated groups, while Sema4D expression was evaluated through RT-qPCR. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (E) Representative protein blots illustrating the expression of Runx2, Osterix, and Col1 in osteoblasts.

Article Snippet: Rabbit anti-Col1 Ab , ABclonal , Cat# A22089;RRID: AB_3665644.

Techniques: Staining, Cell Culture, Derivative Assay, Control, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet: Rv1509 Induces osteoclast differentiation and RANKL secretion by osteoblasts via TLR2 (A) After inducing osteoclast differentiation for four days in the presence of Rv1509 and C29, TRAP staining (scale bar: 400 μm) and F-actin staining (scale bar: 100 μm) were performed, followed by quantitative analysis. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (B) Following a four-day period of osteoclast differentiation stimulated by Rv1509 and C29, RT-qPCR was employed to evaluate the mRNA transcription levels of genes associated with osteoclasts. Additionally, ELISA was performed to measure the expression levels of inflammatory factors. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (C) After 14 days of osteoblast differentiation induced by Rv1509 and C29, ALP staining was performed, followed by Alizarin red staining after 21 days. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (D) The mRNA expression levels of osteoblast-related genes were analyzed using RT-qPCR after 14 days of differentiation in the presence of Rv1509 and C29. The data are shown as mean ± SEM. ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (E) Protein blots showing the representative expression levels of Ctsk, NFATc1, MMP9, and c-Fos in osteoclasts treated with Rv1509 and C29. (F) Protein blots showing representative levels of Col1, Osterix, and Runx2 in osteoblasts after treatment with Rv1509 and C29.

Article Snippet: Rabbit anti-Col1 Ab , ABclonal , Cat# A22089;RRID: AB_3665644.

Techniques: Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Col1 Ab , ABclonal , Cat# A22089;RRID: AB_3665644.

Techniques: Virus, Recombinant, Lysis, Staining, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, BIA-KA, RNA Sequencing, Software